Part:BBa_K2415005:Design

Cry4Aa (Codon optimized for E.coli)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 416
  Illegal BamHI site found at 3496
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1064
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 2508
  Illegal BsaI.rc site found at 3487
  Illegal SapI site found at 2483
  

Design Notes

The sequence was designed to get a better outcome of expression in E.coli.

Source

The target gene fragement was synthesized by IDT.